Review



rabbit polyclonal anxa1 (71–3400) antibody  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher rabbit polyclonal anxa1 (71–3400) antibody
    Source, application and concentration of antibodies.
    Rabbit Polyclonal Anxa1 (71–3400) Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anxa1+(71%E2%80%933400)+antibody/accell+sirnas/pmc10490896-2-0-4
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anxa1 (71–3400) antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches"

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0291023

    Source, application and concentration of antibodies.
    Figure Legend Snippet: Source, application and concentration of antibodies.

    Techniques Used: Concentration Assay, Recombinant, Plasmid Preparation

    Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.
    Figure Legend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Techniques Used: Mass Spectrometry

    Light micrographs show positive immunostaining for PDPN (A), a lymphatic-specific endothelial cell marker, in companion with PGAM1 (B), ENOA1 (C) and ANXA1 (D) in tumor cells. 40X magnification. ANXA1 (E) and PGAM1 (F) colocalize (G) in tumor and endothelial cells in lymphatic intravascular niches. A polarized extravascular tumor cell with redistribution of ANXA1 and PGAM1 into a posterior uropod protrusion (yellow insets) is engaged in amoeboid migration, a hallmark of rapid migration . The maximal projection of confocal images obtained from a z stack (500 nm slice) using a 60x objective (oil) are shown. Overlay images were obtained with transmitted light and excitation at 405 nm (DAPI, blue-violet), 555 nm (ANXA1, green) and 647 nm (PGAM1, red).
    Figure Legend Snippet: Light micrographs show positive immunostaining for PDPN (A), a lymphatic-specific endothelial cell marker, in companion with PGAM1 (B), ENOA1 (C) and ANXA1 (D) in tumor cells. 40X magnification. ANXA1 (E) and PGAM1 (F) colocalize (G) in tumor and endothelial cells in lymphatic intravascular niches. A polarized extravascular tumor cell with redistribution of ANXA1 and PGAM1 into a posterior uropod protrusion (yellow insets) is engaged in amoeboid migration, a hallmark of rapid migration . The maximal projection of confocal images obtained from a z stack (500 nm slice) using a 60x objective (oil) are shown. Overlay images were obtained with transmitted light and excitation at 405 nm (DAPI, blue-violet), 555 nm (ANXA1, green) and 647 nm (PGAM1, red).

    Techniques Used: Immunostaining, Marker, Migration

    Related Articles

    other:

    Article Title: New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination
    Article Snippet: ANXA1 expression analysis by RT-qPCR using the TaqMan method Total RNA was extracted from the cultured cells (SK-MEL-3, SK-MEL-28, A375-MA2) according to the manufacturer’s instructions using an RNeasy mini kit (Qiagen).

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches
    Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

    Article Title: Inhibition of the AnxA1/FPR1 autocrine axis reduces MDA-MB-231 breast cancer cell growth and aggressiveness in vitro and in vivo.
    Article Snippet: TMA slides were incubated overnight with anti-AnxA1 (rabbit polyclonal; 71-3400, Invitrogen) at 1:500 dilution or with anti-FPR1 (rabbit polyclonal; SAB4501007, Sigma) at 1:50 dilution.

    Article Title: Inhibition of the AnxA1/FPR1 autocrine axis reduces MDA-MB-231 breast cancer cell growth and aggressiveness in vitro and in vivo.
    Article Snippet: Cells were stained with anti-AnxA1 (rabbit polyclonal; 1:200; 71-3400, Invitrogen), anti-FPR1 (rabbit polyclonal; 1:100; SAB4501007, Sigma) and anti-FPR2 (rabbit ACCEPTED MANUSCRIPT AC C EP TE D M AN U SC R IP T 9 polyclonal; 1:100; HPA029154, Sigma) for membrane expression evaluation.

    Article Title: New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination
    Article Snippet: ANXA1 level analysis by flow cytometry SK-MEL-3, SK-MEL-28 and A375-MA2 cells were harvested at a number of 1×10 6 and stained with anti-ANXA1 (rabbit polyclonal, 1:500 for permeabilized and 1:100 for non-permeabilized cells, 71-3400, Invitrogen) to evaluate total and membrane expression.

    Immunocytochemistry:

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches
    Article Snippet: Mouse monoclonal PGAM1 (67470-1-1g) ThermoFisher Scientific (1:200) , ICC/IF: Goat anti-mouse, Alexa FluorTM Plus 647 (A-32728) ThermoFisher Scientific (~4 μg/mL). .. Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1 μg/mL) , ICC/IF: Donkey anti-rabbit, Alexa FluorTM 555 (A-31572) ThermoFisher Scientific (~4 μg/mL). ..

    Staining:

    Article Title: New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination
    Article Snippet: A semi-quantitative measurement of protein level was obtained using Image Lab software (Bio-Rad). .. SK-MEL-3, SK-MEL-28 and A375-MA2 cells were harvested at a number of 1×10 6 and stained with anti-ANXA1 (rabbit polyclonal, 1:500 for permeabilized and 1:100 for non-permeabilized cells, 71-3400, Invitrogen) to evaluate total and membrane expression. .. The following primers and TaqMan probes, dye-labeled with FAM-MGB (VIC-MGB for UBC), were used in the reaction: Hs00167549_m1 for ANXA1 and Hs01871556_s1 for UBC .

    Membrane:

    Article Title: New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination
    Article Snippet: A semi-quantitative measurement of protein level was obtained using Image Lab software (Bio-Rad). .. SK-MEL-3, SK-MEL-28 and A375-MA2 cells were harvested at a number of 1×10 6 and stained with anti-ANXA1 (rabbit polyclonal, 1:500 for permeabilized and 1:100 for non-permeabilized cells, 71-3400, Invitrogen) to evaluate total and membrane expression. .. The following primers and TaqMan probes, dye-labeled with FAM-MGB (VIC-MGB for UBC), were used in the reaction: Hs00167549_m1 for ANXA1 and Hs01871556_s1 for UBC .

    Expressing:

    Article Title: New insight into the role of ANXA1 in melanoma progression: involvement of stromal expression in dissemination
    Article Snippet: A semi-quantitative measurement of protein level was obtained using Image Lab software (Bio-Rad). .. SK-MEL-3, SK-MEL-28 and A375-MA2 cells were harvested at a number of 1×10 6 and stained with anti-ANXA1 (rabbit polyclonal, 1:500 for permeabilized and 1:100 for non-permeabilized cells, 71-3400, Invitrogen) to evaluate total and membrane expression. .. The following primers and TaqMan probes, dye-labeled with FAM-MGB (VIC-MGB for UBC), were used in the reaction: Hs00167549_m1 for ANXA1 and Hs01871556_s1 for UBC .

    Incubation:

    Article Title: Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness
    Article Snippet: .. 48 Proteins were visualized using the chemioluminescence detection system (Amersham) after incubation with primary antibodies against ANXA1 (rabbit polyclonal; 1:10000; 71–3400, Invitrogen), α-tubulin (mouse monoclonal; clone DM1A; 1:1000; Sigma-Aldrich), E-cadherin (mouse monoclonal; clone 36/E-Cadherin; 1:10000; BD Transduction Laboratories), vimentin (mouse monoclonal; clone E-5; 1:1000; Santa Cruz Biotechnologies), HIF1-α (rabbit polyclonal; clone A300–286A; 1:5000; Bethyl Laboratories), Ezrin (mouse monoclonal; clone 3C12; 1:250; Santa Cruz Biotechnologies), Moesin (mouse monoclonal; clone E-10; 1:250; Santa Cruz Biotechnologies), CK8 (mouse monoclonal, clone M20; 1:1000; Santa Cruz Biotechnologies), CK18 (mouse monoclonal, clone C-04; 1:1000; Santa Cruz Biotechnologies), p-ERK (rabbit monoclonal, clone Aw39; 1:1000; Cells Signaling), ERK (mouse monoclonal, clone MK12; Cells Signaling), p-p38 (mouse monoclonal; clone D-8; 1:1000; Santa Cruz Biotechnologies), p38 (rabbit polyclonal; clone C-20; 1:1000; Santa Cruz Biotechnologies), β-actin (mouse monoclonal; clone C-4, 1:1000; Santa Cruz Biotechnologies). .. The blots were exposed to Las4000 (GE Healthcare Life Sciences) and the relative band intensities were determined using ImageQuant software (GE Healthcare Life Sciences).



    Similar Products

    90
    Thermo Fisher rabbit polyclonal anxa1 (71–3400) antibody
    Source, application and concentration of antibodies.
    Rabbit Polyclonal Anxa1 (71–3400) Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anxa1+(71%E2%80%933400)+antibody/accell+sirnas/pmc10490896-2-0-4
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anxa1 (71–3400) antibody - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Source, application and concentration of antibodies.

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Source, application and concentration of antibodies.

    Article Snippet: Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1 μg/mL) , ICC/IF: Donkey anti-rabbit, Alexa FluorTM 555 (A-31572) ThermoFisher Scientific (~4 μg/mL).

    Techniques: Concentration Assay, Recombinant, Plasmid Preparation

    Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Article Snippet: Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1 μg/mL) , ICC/IF: Donkey anti-rabbit, Alexa FluorTM 555 (A-31572) ThermoFisher Scientific (~4 μg/mL).

    Techniques: Mass Spectrometry

    Light micrographs show positive immunostaining for PDPN (A), a lymphatic-specific endothelial cell marker, in companion with PGAM1 (B), ENOA1 (C) and ANXA1 (D) in tumor cells. 40X magnification. ANXA1 (E) and PGAM1 (F) colocalize (G) in tumor and endothelial cells in lymphatic intravascular niches. A polarized extravascular tumor cell with redistribution of ANXA1 and PGAM1 into a posterior uropod protrusion (yellow insets) is engaged in amoeboid migration, a hallmark of rapid migration . The maximal projection of confocal images obtained from a z stack (500 nm slice) using a 60x objective (oil) are shown. Overlay images were obtained with transmitted light and excitation at 405 nm (DAPI, blue-violet), 555 nm (ANXA1, green) and 647 nm (PGAM1, red).

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Light micrographs show positive immunostaining for PDPN (A), a lymphatic-specific endothelial cell marker, in companion with PGAM1 (B), ENOA1 (C) and ANXA1 (D) in tumor cells. 40X magnification. ANXA1 (E) and PGAM1 (F) colocalize (G) in tumor and endothelial cells in lymphatic intravascular niches. A polarized extravascular tumor cell with redistribution of ANXA1 and PGAM1 into a posterior uropod protrusion (yellow insets) is engaged in amoeboid migration, a hallmark of rapid migration . The maximal projection of confocal images obtained from a z stack (500 nm slice) using a 60x objective (oil) are shown. Overlay images were obtained with transmitted light and excitation at 405 nm (DAPI, blue-violet), 555 nm (ANXA1, green) and 647 nm (PGAM1, red).

    Article Snippet: Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1 μg/mL) , ICC/IF: Donkey anti-rabbit, Alexa FluorTM 555 (A-31572) ThermoFisher Scientific (~4 μg/mL).

    Techniques: Immunostaining, Marker, Migration